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King Tut DNA testing sheds light on how he lived and died

The first successful DNA testing of Egyptian mummies -- including on that of King Tut -- sheds light on what caused the boy king's death, and what led to his problems in life.

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Amr Nabil/AP
Women look at one of the coffins of King Tutankhamun Monday at the Egyptian museum in Cairo. Egypt's famed King Tutankhamun suffered from a cleft palate and club foot, likely forcing him to walk with a cane, and died from complications from a broken leg exacerbated by malaria, according to the most extensive study ever of his mummy.

The results of a study on the mummy of King Tutankhamun, Egypt鈥檚 golden-boy pharaoh, released in Cairo this week appeared to disprove the popular theory that King Tut was murdered.

Instead, researchers said, their tests showed King Tut died at age 19 due to a combination of bad genes, bad bones and a bad case of malaria.

The two-year study was the first to use advanced radiological and genetic testing techniques on mummies, originally thought impossible due to their age and the techniques used to preserve the bodies. The team of Egyptian and foreign scientists led by Zahi Hawass, secretary general of the Egyptian Supreme Council of Antiquities, extracted DNA from the bones of eleven mummies and published their findings in the Journal of the American Medical Association on Wednesday.

鈥淭his is the first time somebody did something like this with pharaohnic DNA,鈥 says Carsten Pusch associate professor at the Institute of Human Genetics at the University of T眉bingen in Germany, who worked on the study. 鈥淲e have shown it is possible to work with the DNA [of mummies] and now we have opened a new door. Behind the door, there鈥檚 a new universe waiting for us.鈥

Using CAT scans and DNA analysis the study found the boy-pharaoh, who died in the ninth year of his reign in 1324 BC, had a cleft palette, a club foot, avascular bone necrosis in his left foot (a disease that prevents adequate blood flow to the bone), and walked with a cane as a result 鈥 explaining the approximately 130 walking sticks found in Tut鈥檚 tomb, discovered by British archeologist Howard Carter in 1928.

DNA testing pointed to incestuous parentage as at the root of Tut鈥檚 health woes. The study concluded his father was the controversial King Akhenaten, who attempted to bring monotheism to ancient Egypt, while Tut鈥檚 mother was Akhenaten鈥檚 sister, whose name remains unknown. Incest was normal in ancient Egypt, but often led to chronic health problems in the ruling families.

Hawass declares cause of death

鈥淣ow we can finally answer the question of how King Tut died,鈥 said Hawass. 鈥淜ing Tut had severe malaria.鈥 Testing also showed one of the two fetuses found in Tut鈥檚 tomb was his 9-month-old daughter.

Ancient DNA research is not just being done in Egypt. Last week, scientists using similar methods announced they had sequenced the DNA of "Inuk" a 4,000-year-old man who lived in what is now Greenland and found his ancestors had traveled there from Siberia.

鈥淲ith the methods 10, 15 years ago, it would be impossible to do this kind of research,鈥 says Dr. Pusch, who notes the superiority of man-made enzymes today allowed these studies to move forward. 鈥淚t鈥檚 just some kind of cooking in the lab, but you need many, many ingredients, and these ingredients are of perfect high quality now.鈥

Despite new technology, scientists were not sure the mummification process would preserve DNA well enough to test. 鈥淚t is very important to see if it is generally possible to work with old specimens that have been embalmed 鈥 this is completely different from specimens that have been buried in the soil or frozen in the ice 鈥 with this study we have shown it is possible,鈥 says Pusch.

Could a pharaoh be cloned? 鈥淚t was shown it was possible for the Neanderthal, and the Neanderthal is even older, so it should be possible with these,鈥 he says.

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